A cell aggregate is aspired through a constriction
In a nutshell
An aggregate of cells is forced through a hole. This allows us to study its mechanical properties.
A few details
This is an aggregate of mouse embryo carcinoma cells (F9 cells). The hole is 50 by 140 microns (and 100 microns in the direction perpendicular to the image). The cells are labeled with sulforhodamine B, and images are taken by two-photon microscopy. A known pressure difference is applied between the left and right compartments. Passage through the constriction leads to large deformations of the aggregate, which can be measured. This experiment enables in situ measurements of the local viscoelastic properties of a 3D tissue, and the link with intra- or intercellular events, such as shape changes and rearrangements.
Video produced by Sham Tlili during her thesis
Collaboration MSC and Institut Lumière-Matière (Lyon)
Reference
Scientific article: S. Tlili, F. Graner, H. Delanoë-Ayari “A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization”, Development, 149 (2022)
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